{"id":4120,"date":"2024-02-21T15:16:40","date_gmt":"2024-02-21T20:16:40","guid":{"rendered":"https:\/\/purity-iq.com\/?page_id=4120"},"modified":"2024-03-07T14:20:58","modified_gmt":"2024-03-07T19:20:58","slug":"qpcr-and-viability-pcr","status":"publish","type":"page","link":"https:\/\/purity-iq.com\/qpcr-and-viability-pcr\/","title":{"rendered":"qPCR and Viability PCR"},"content":{"rendered":"
qPCR and Viability PCR<\/strong><\/p>\n We use qPCR for probiotic identification and viability PCR for viable cell count.<\/p>\n <\/span><\/span><\/span><\/p>\n qPCR<\/strong><\/p>\n qPCR is a strong DNA-based analytical tool that can detect single strains in multiple component probiotic blends. For identification, our method allows us to do strain specific identification versus other methodologies that only do species level identification. This is important because probiotic health benefits are strain specific.<\/p>\n Viability PCR<\/strong><\/p>\n For viable cell count testing, we use viability PCR. It is sensitive, fast, and accurate. It is also strain specific and can accurately quantify the individual viable microorganisms in a multi-ingredient blended product. For a probiotic to be effective and confer a health benefit, the strain needs to be live and at an expected concentration.<\/p>\n <\/span><\/span><\/p>\n Probiotic Health Benefits are Strain Specific<\/strong><\/p>\n Probiotic health benefits are strain specific and dose dependent. Therefore authenticity testing for probiotics requires reliable methods that can achieve strain-level identification and viable cell count of multi-strain probiotic products.<\/p>\n \n In qPCR, a specific sequence region of DNA is targeted and amplified to rapidly create millions of copies of this region to make it detectable. Methods are developed for each target probiotic strain to ensure specificity and no cross reactivity with closely related strains, making it ideal to identify target strains in raw ingredients as well as in multi-strain finished products.<\/p>\n Genus, Species and Strain<\/strong><\/p>\n <\/span><\/p>\n Why Viable Cell Count Is Essential for Quality Probiotic Products<\/strong><\/p>\n Viable cell count is a crucial step in the evaluation of probiotic products. By accurately quantifying the number of viable microorganisms in a probiotic sample, several product aspects can be assured: product quality, efficacy, production, comparison, and regulatory compliance.<\/p>\n \n Viable cell count refers to the process of quantifying the number of viable microorganisms <\/strong>in a sample of a probiotic product. The number of live bacteria present in the product can have a significant impact on its therapeutic benefits. <\/p>\n <\/span><\/p>\n Here are some of the key reasons why viable cell count is important in probiotics:<\/strong><\/p>\n Ensuring product quality and efficacy<\/strong><\/p>\n Confirms product label claims<\/strong><\/p>\n Identifying potential production issues<\/strong><\/p>\n Ensuring regulatory compliance<\/strong><\/p>\n Enabling comparison between products<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":" […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"Matt Barth","author_link":"https:\/\/purity-iq.com\/author\/matt-editor\/"},"uagb_comment_info":0,"uagb_excerpt":"[…]","_links":{"self":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/4120"}],"collection":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/comments?post=4120"}],"version-history":[{"count":32,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/4120\/revisions"}],"predecessor-version":[{"id":5089,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/4120\/revisions\/5089"}],"wp:attachment":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/media?parent=4120"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n