{"id":2687,"date":"2023-02-28T22:47:51","date_gmt":"2023-03-01T03:47:51","guid":{"rendered":"https:\/\/purity-iq.com\/?page_id=2687"},"modified":"2023-08-08T08:04:53","modified_gmt":"2023-08-08T12:04:53","slug":"form-page-probiotic-strain-level-viable-count-using-viability-pcr","status":"publish","type":"page","link":"https:\/\/purity-iq.com\/form-page-probiotic-strain-level-viable-count-using-viability-pcr\/","title":{"rendered":"Form Page &#8211; Probiotic Strain Level Viable Count Using Viability PCR"},"content":{"rendered":"<p style=\"\">Probiotic Strain Level Viable Count Using Viability PCR<\/p>\n<p style=\"\">This application note presents the development and validation of a viability real-time PCR (v-PCR) method for strain-specific enumeration of <em>Lacticaseibacillus rhamnosus <\/em>GG in probiotic products. Fill out the form to learn more about how the v-PCR method offers high specificity, fast and accurate results, and the ability to quantify viable but non culturable (VBNC) cells, providing a valuable tool for quality control and compliance in probiotic manufacturing.<\/p>\n<p><span><img decoding=\"async\" alt=\"\" data-id=\"3192\" width=\"442\" data-init-width=\"2000\" height=\"442\" data-init-height=\"2000\" title=\"Viability PCR cover photo\" loading=\"lazy\" src=\"https:\/\/purity-iq.com\/wp-content\/uploads\/2023\/08\/Viability-PCR-cover-photo.png\" data-width=\"442\" data-height=\"442\" style=\"aspect-ratio: auto 2000 \/ 2000;\"><\/span><\/p>\n<p style=\"\">What Will You Learn?<\/p>\n<ul>\n<li><span>The rapid growth of the global probiotic market and the importance of verifying probiotic product label claims and viable cell counts for efficacy.<\/span><\/li>\n<li><span>The drawbacks of traditional plate count methods for viable count determination and the advantages of viability real-time PCR (v-PCR) in quantifying viable but non-culturable (VBNC) cells.<\/span><\/li>\n<li><span>The development and validation of a strain-specific v-PCR method for <em>Lacticaseibacillus rhamnosus<\/em> GG, including the selection of an efficient viability dye treatment and DNA liberation protocol.<\/span><\/li>\n<li><span>The performance comparison between the v-PCR method and the plate count method for viable count determination of <em>L. rhamnosus<\/em> GG in both mono-strain and multi-strain samples, with applicability to samples containing non-active ingredients.<\/span><\/li>\n<\/ul>\n<p><span><img decoding=\"async\" alt=\"\" data-id=\"3207\" width=\"520\" data-init-width=\"1430\" height=\"272\" data-init-height=\"748\" title=\"Picture 4\" loading=\"lazy\" src=\"https:\/\/purity-iq.com\/wp-content\/uploads\/2023\/08\/Picture-4.jpg\" data-width=\"520\" data-height=\"272\" style=\"aspect-ratio: auto 1430 \/ 748;\"><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p> [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false},"uagb_author_info":{"display_name":"Matt Barth","author_link":"https:\/\/purity-iq.com\/author\/matt-editor\/"},"uagb_comment_info":0,"uagb_excerpt":"[&hellip;]","_links":{"self":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/2687"}],"collection":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/comments?post=2687"}],"version-history":[{"count":4,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/2687\/revisions"}],"predecessor-version":[{"id":3233,"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/pages\/2687\/revisions\/3233"}],"wp:attachment":[{"href":"https:\/\/purity-iq.com\/wp-json\/wp\/v2\/media?parent=2687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}