Probiotic Strain Level Viable Count Using Viability PCR

This application note presents the development and validation of a viability real-time PCR (v-PCR) method for strain-specific enumeration of Lacticaseibacillus rhamnosus GG in probiotic products. Fill out the form to learn more about how the v-PCR method offers high specificity, fast and accurate results, and the ability to quantify viable but non culturable (VBNC) cells, providing a valuable tool for quality control and compliance in probiotic manufacturing.

What Will You Learn?

  • The rapid growth of the global probiotic market and the importance of verifying probiotic product label claims and viable cell counts for efficacy.
  • The drawbacks of traditional plate count methods for viable count determination and the advantages of viability real-time PCR (v-PCR) in quantifying viable but non-culturable (VBNC) cells.
  • The development and validation of a strain-specific v-PCR method for Lacticaseibacillus rhamnosus GG, including the selection of an efficient viability dye treatment and DNA liberation protocol.
  • The performance comparison between the v-PCR method and the plate count method for viable count determination of L. rhamnosus GG in both mono-strain and multi-strain samples, with applicability to samples containing non-active ingredients.
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