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The Growing Popularity of Mushroom Products and the Importance of Testing Protocols

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The Growing Popularity of Mushroom Products and the Importance of Testing Protocols

Mushrooms are a trending ingredient in the health and wellness market. They are gaining in popularity due to a wide range of potential health benefits: these fungi possess possible anti-inflammatory, antimicrobial, brain, heart and health benefits, as well as immune and energy boosting properties.

Consumers are increasingly seeking out mushrooms as a daily supplement. While mushrooms have been used by our ancestors for millennia to treat all sorts of diseases, today’s health- conscious consumer looks for a convenient diet supplement.  Mushroom products are available in various forms, including powders, capsules, and extracts. Several mushroom varieties can be found in wellness products including Reishi (Ganoderma), Hericium (Lion’s Mane), Shiitake, Cordyceps, and Turkey Tail.

As with all supplements, consumers purchase products with the expectation of a health benefit, and assurance that the ingredients stated on the package match the ingredients in the product. Companies with transparent quality assurance practices employ testing protocols to provide this.

One of the biggest problems regarding testing for mushroom products is the accurate identification of the species of mushroom. There are many different species of mushrooms and even experts can have difficulty distinguishing between different species of mushrooms and it’s not limited to visual identification.

Another challenge in testing for mushroom products is the variability in the levels of active compounds, such as polysaccharides, terpenoids, and triterpenoids, that can be present in different samples of the same species of mushroom. This can make it difficult to establish consistent quality control standards for mushroom products and to accurately measure the potency of medicinal or nutritional compounds in these products.

Nuclear Magnetic Resonance (NMR) is an accurate and reliable testing method for mushrooms.

NMR is a non-targeted and inherently quantitative analytical technique that is commonly used in chemistry and biology to determine the chemical composition of a sample.

NMR can identify the presence and concentration of specific molecules in a sample, providing detailed information on the product's composition. NMR works by placing a sample in a strong magnetic field, which causes the nuclei of the atoms in the sample to align. A pulse of radiofrequency energy is then applied to the sample, causing the nuclei to resonate and emit a signal that can be detected and analyzed. This signal provides information about the sample's chemical composition, allowing spectroscopists to identify the presence of specific molecules and the concentration of each component.

 It is also a powerful testing method for a few reasons:

  • Unlike traditional testing methods such as chromatography, NMR analysis can provide a wealth of information about the molecular composition of a product. This is because NMR detects the magnetic properties of the atomic nucleus, providing information about the chemical environment of the atoms within the molecule. 
  • It can be used to study the composition of complex mixtures without prior knowledge of their components.
  • Investing in NMR analysis allows companies to build a comprehensive library of reference spectra that can be used for comparison with future samples, ensuring the safety and consistent quality of their products.

NMR Testing: An Essential Tool in Research and Development Protocols

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NMR Testing: An Essential Tool in Research and Development Protocols

Healthy brands and businesses are frequently refining the ingredients and components in their products. A company’s research and development (R&D) team may propose new or enhanced ingredients to a product for several reasons: 

  • Improved quality
  • Lower cost
  • Better availability
  • Improved Performance
  • Improved Regulations

Often products are made up of numerous ingredients sourced from multiple suppliers. This can make it challenging to ensure that each ingredient is of the highest quality and free from adulterants. In the worst of cases, a new, untested ingredient in a product can cause adulteration. If discovered, a recall can occur. Recalls can be major setbacks for any business: they require immediate action and communication may result in an on-going impact to a brand’s reputation.  

For valuable brands, new ingredient testing should be part of all research and development protocols when any product formulation is revised. By investing in this important stage of R&D, brands can ensure the authenticity of all ingredients and formulations and identify the optimal composition of raw ingredients and finished products.  

In this context, NMR (Nuclear Magnetic Resonance) is a valuable tool for R&D.  NMR is a non-targeted analytical technique that uses a magnetic field and radiofrequency radiation to examine the molecular structure and composition of a sampleIt can provide a great deal of information such as:  

  • Detailed information about the chemical composition of a product
  • The identity of chemical compounds present in a sample
  • The quantification of compounds present in a sample
  • The detection of contaminants or adulterants

It is also a powerful testing method for a few reasons:

  • Unlike traditional testing methods such as chromatography, NMR analysis can provide a wealth of information about the molecular composition of a product. This is because NMR detects the magnetic properties of the atomic nucleus, providing information about the chemical environment of the atoms within the molecule.   
  • It can be used to study the molecular structure and composition of complex mixtures without prior knowledge of their components.
  • Investing in NMR analysis allows companies to build a comprehensive library of reference spectra that can be used for comparison with future samples, ensuring the safety and consistent quality of their products.  

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3 Types of Elderberry Adulteration and How to Detect Them 

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3 Types of Elderberry Adulteration and How to Detect Them 

Elderberry has gained significant attention recently due to its immune-boosting health benefits. This powerful plant, belonging to the genus Sambucus and native to Europe, has been the subject of numerous scientific studies that have found it to be effective in reducing the severity and duration of viral infections. 

While the demand for elderberry continues to grow, the problem of adulteration has become a significant concern for brand owners and ingredient suppliers alike. Adulteration refers to the addition of lower-quality or artificial ingredients to elderberry products. This can potentially harm consumers seeking the health benefits associated with elderberry. 

The high demand for elderberry has led to a supply shortage in some regions. This has resulted in using cheaper alternatives, such as black mulberry or black currant, to create elderberry products. 

The problem of adulteration not only affects the credibility of the suppliers but also poses a significant risk to consumers who may unknowingly consume products that contain harmful substances or lack the expected health benefits. This can also negatively impact the reputation of suppliers and brand owners who prioritize the quality and authenticity of their products. 

In this blog, we will delve deeper into the issue of elderberry adulteration, discussing the extent of the problem and its impact on the industry. We will discuss the current state of Elderberry adulteration and how brand owners and ingredient suppliers can ensure the authenticity of their products through Nuclear Magnetic Resonance (NMR). So, if you want to learn more about this important issue, read on! 

The State of Elderberry Adulteration 

Elderberry adulteration is a widespread issue in the industry that has become increasingly concerning in recent years. The current state of adulteration involves the addition of lower-quality or artificial ingredients to elderberry products, such as juices, syrups, and extracts. 

There are several different forms of elderberry adulteration, including: 

  • The use of cheaper substitutes, such as black mulberry and black currant, which lack the same health benefits as authentic elderberries.
  • The dilution of elderberry extracts with other substances, such as water, to reduce costs which can compromise the product's potency, safety, and quality. 

  • Adding artificial flavours and colours to elderberry products can mask the lack of authentic elderberry content. 

Elderberry adulteration can significantly impact the reputation and revenue of brands prioritizing their products' quality and authenticity. When consumers discover that a brand has been selling adulterated elderberry products, their trust and confidence in the brand can be severely undermined. This decline in trust can significantly impact a brand's reputation. Consumers who no longer trust a brand may switch to competitors or stop purchasing elderberry products altogether, resulting in a decline in sales and revenue and a loss of market share. 

On the other hand, a brand known for producing high-quality and authentic elderberry products can earn a positive reputation and increase brand loyalty. To take it a step further, a brand that is taking the necessary steps to ensure the purity and authenticity of their elderberry products will be eligible for third-party certifications, providing them with a competitive advantage that can be marketed to consumers. 

The Role of Nuclear Magnetic Resonance (NMR) in Detecting Elderberry Adulteration

There are several testing methods currently used to detect adulteration in elderberry products. However, each method has its limitations and may only be effective in detecting some types of adulteration. 

The following are the most commonly used testing methods for detecting elderberry adulteration: 

  • DNA Testing: DNA testing is targeted and can detect the presence of black currant and black mulberry DNA in elderberry products. While this method can accurately identify the presence of these substitutes, it may require more work to detect the addition of other cheaper substitutes.  
  • HPLC: High-performance liquid chromatography (HPLC) measures the levels of specific compounds in elderberry extracts. This method could identify dilution and substitution with other berry extracts but may not be able to detect all kinds of adulterants. 

  • Spectrophotometry: Spectrophotometry is a method that measures the absorbance of light by a substance to determine its concentration. It can detect the presence of elderberry anthocyanins, which are specific pigments found in elderberry. However, this method cannot detect the presence of cheaper substitutes or dilution with other ingredients. 

Nuclear Magnetic Resonance (NMR) spectroscopy is one of the most accurate and reliable methods for detecting adulteration in elderberry products. NMR can identify the presence and concentration of specific molecules in a sample, providing detailed information on the product's composition. 

NMR works by placing a sample in a strong magnetic field, which causes the nuclei of the atoms in the sample to align. A pulse of radiofrequency energy is then applied to the sample, causing the nuclei to resonate and emit a signal that can be detected and analyzed. This signal provides information about the sample's chemical composition, allowing spectroscopists to identify the presence of specific molecules and the concentration of each component. 

One of the main advantages of NMR is its ability to identify a wide range of molecules and compounds, including complex mixtures. This makes it a highly effective method for detecting the addition of cheaper substitutes or other adulterants and the dilution of elderberry extracts. In addition, NMR can be used to verify the authenticity of elderberry extracts by identifying specific markers unique to elderberry. 

NMR is the best option for detecting adulteration in elderberry products due to its accuracy, reliability, and ability to detect a wide range of molecules and compounds. Its ability to provide detailed information on the composition of a sample makes it an essential tool for brand owners and ingredient suppliers who prioritize the quality and authenticity of their products.  

Four Ways that Strain Specific Enumeration can Increase Operational Efficiencies for Probiotic Products

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Four Ways that Strain Specific Enumeration can Increase Operational Efficiencies for Probiotic Products

Strain specific enumeration can significantly improve operational efficiencies in the production of probiotic products. Enumeration refers to the process of quantifying the number of viable microorganisms in a given sample of a probiotic product. The number of live bacteria present in the product can have a significant impact on its therapeutic benefits.

Strain specific enumeration can improve operational practices in the probiotic industry in these four ways:

  1. 1
    Improved batch-to-batch consistency: by enumerating the number of viable microorganisms in a probiotic product, manufacturers can ensure that each batch contains a consistent number of microorganisms. This can help to improve batch-to-batch consistency and reduce the variability between different batches of the same product.
  2. 2
    Faster product release: strain specific enumeration can help manufacturers release their products more quickly. By confirming that a product meets the required levels of live microorganisms, manufacturers can ensure the product is safe and effective, and ready to release to the market.
  3. 3
    Better product development and optimization: strain specific enumeration can help manufacturers to develop and optimize their products more effectively. By accurately measuring the number of live microorganisms in a product, manufacturers can determine the appropriate dosage needed to achieve the desired health effects.
  4. 4
    Reduced production costs: strain specific enumeration can also help to reduce production costs. By accurately measuring the number of viable microorganisms in a probiotic product, manufacturers can ensure that they are using the right amount of raw materials and reduce wastage.

There are several methods used for the enumeration of probiotics, including plate count methods, flow cytometry, and molecular techniques such as viability-qPCR (v-PCR).

Innovative strain specific enumeration with viability qPCR is used for the analysis of raw material to all different dosage forms of probiotic products for accurate determination of viable counts throughout the shelf life.

Purity-IQ is a leader in strain specific enumeration services and offers enumeration analysis that can be used for single or multi-strain blends, strain specific counts, tyndallized (heat-killed), and next generation probiotics.

Mushroom Species Identification and Consistency Using NMR

Mushroom Species Identification and Consistency Using NMR

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Differentiating Elderberry Products Using NMR Metabolomics

Differentiating Elderberry Products Using NMR Metabolomics

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Curcuma Species Identification and Adulterant Detection

Curcuma Species Identification and Adulterant Detection

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Protecting Your Brand With Science-Based Solutions

Protecting Your Brand With Science-Based Solutions

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Developing a Leading Probiotic Offer Through Advanced Analytics

Probiotic Consistency Product Analysis

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Probiotic Consistency Product Analysis

Probiotic Consistency Product Analysis

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